Skip to main content
. 2021 Sep 1;131(17):e145472. doi: 10.1172/JCI145472

Figure 7. RNF186-dependent UPR signaling is required for NOD2-induced bacterial clearance and antimicrobial pathways.

Figure 7

(A–D) MDMs (n = 6) were transfected with scrambled or RNF186 siRNA, then treated with 100 μg/mL MDP for 48 hours ± 10 μM CPA. (E–H) MDMs (n = 6; similar results in an additional n = 6) were transfected with empty vector (EV) or FLAG-tagged WT or lysine mutants (K149A, K152A, or K356A) of ATF6 and then treated with 100 μg/mL MDP for 48 hours. (I–L) MDMs (n = 6) (rs6426833 AA low RNF186-expressing carriers) were transfected with EV or myc-tagged RNF186-A64 (WT) or RNF186-T64 (risk variant) and then treated with 100 μg/mL MDP for 48 hours ± 10 μM CPA. (A, E, and I) Bacterial uptake. (B, F, and J) Intracellular bacterial clearance. (C, G, and K) ROS production. (D, H, and L) LC3II and ATG5 expression. Mean + SEM. *P < 0.05; **P < 0.01; ***P < 0.001; †P < 1 × 10−4; ††P < 1 × 10−5 determined by 2-tailed Student’s t test with a Bonferroni-Holm correction for multiple comparisons. Scr, scrambled; Vec, vector.