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. 2021 Sep 1;16(9):e0256440. doi: 10.1371/journal.pone.0256440

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© 2021 Abe et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) The ARID1A gene was knocked out in the gastric cancer cell line MKN74 using CRISPR Cas9. Two ARID1A-knockout clones show a fibroblastic morphology of various degrees. (B) RT-PCR of BZLF1 gene of EBV, showing absence of expression in both EBV-infected parental MKN74 cells and ARID1A-knockout clones. (C) Parental MKN74 cells and ARID1A-knockout MKN74 clones #1 and #2 were infected with GFP-integrated cell-free EBV. Several ARID1A-knockout cells showed green fluorescence on Day 2. (D) Flow cytometry analyses of fluorescent-positive cells on Day 2. SSC, side scatter; FITC, fluorescein isothiocyanate.