Skip to main content
. 2021 Aug 20;17(8):e1009868. doi: 10.1371/journal.ppat.1009868

Fig 6. Transfer of B cell-bound EBV particles to NK cells is encompassed by trogocytosis.

Fig 6

(A-E) B cells were coated with B95.8 VP (A-C) or AKBM VP (D, E), co-cultured with NK cells and EBV S- or S+ serum for the indicated times, and stained for CD21, CD19, CD20 and CD56. The proportions of CD21+ (A, D), CD19+ (B, E) and CD20+ (C) NK cells (gated as in Fig 4A) are shown. Representative plots of EBV S- at 4h and EBV S+ at 15min (left) and of four (A-B) and three (C-E) experiments (right) are displayed. Statistical analysis was performed with Friedman test with Dunn’s multiple comparison test. (F) B cells were stained with 2μM PKH26, coated with B95.8 VP and co-cultured for the indicated times with NK cells and EBV S- or S+. Proportions of PKH26+ NK cells in representative plots of EBV S- at 4h and EBV S+ at 15min (left) and of three experiments are shown. (G, H) B cells were treated and co-cultured as in (F) but for 4h in the presence of EBV S-, EBV S+ or 12.5 ng/ml of Rituximab. The proportions of CD19+ (E) and PKH26+ (F) NK cells corresponding to two experiments are shown.