Fig 7. Adoptively transferred T cells from Luteolin-treated mice confer improved protection against TB.

(A) Experimental Layout: CD4⁺ T cells were isolated from congenic wild-type Thy1.2⁺ mice infected with H37Rv followed by Luteolin treatment for 120 days and rested for 30 days. CD4⁺ T cells (10x10⁶) were adoptively transferred into γ-irradiated (sub-lethal dose of 800 rads/mice) Thy1.1⁺ congenic animals followed by infection with H37Rv. Twenty days after infection, spleen and lungs were isolated. (B) CFUs were estimated from lung homogenates of the different groups. (C) Splenocytes were challenged with H37Rv complete soluble antigen ex vivo. T cells were then stained for the intracellular cytokines IL-4 vs. IFN-γ, and IL-17. The results shown are representative of one experiment with six mice within each group, of which only 4 mice in the control group and 3 mice in the ISONIAZID AT group and 5 mice in the LUTEOLIN + ISONIAZID AT group survived by day 20. Differences were considered significant at P<0.05 and are represented by * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 whereas non-significant differences are denoted by (NS).