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UBP43 encodes a functional deubiquitinating enzyme. A ubiquitin–β-galactosidase (Ub-Met-β-gal) fusion protein expressed in bacteria was deubiquitinated. Shown is a Western blot with anti-β-galactosidase antiserum. Coexpressed plasmids were pGEX-4T-3-UBP43 (lanes 1 to 4), the positive control pGEX-DUB-2 (lane 5), and the negative control pGEX-4T-3 (vector) (lane 6).
