TABLE 1.
Induction of terminal differentiation in control M1 cells and UBP43-overexpressing M1 cells
| Cells used (treatment)a | Cell type (%)b
|
||
|---|---|---|---|
| Blast | Intermediate | Mature | |
| neo/M1 (IL-6) | 14.7 ± 0.76 | 32.5 ± 2.4 | 52.8 ± 2.2 |
| UBP43/M1 (IL-6) | 65.3 ± 1.7 | 29.2 ± 0.9 | 5.5 ± 2.5 |
| neo/M1 (LIF) | 10.7 ± 1.75 | 39.3 ± 2.7 | 50 ± 1.1 |
| UBP43/M1 (LIF) | 67.4 ± 2.7 | 27.9 ± 3.1 | 4.7 ± 2.5 |
M1 cells infected with a retroviral vector containing UBP43 cDNA (UBP43/M1) were compared to control M1 cells infected with a retroviral vector alone (neo/M1) after culture in the presence of IL-6 (100 ng/ml) or LIF (50 ng/ml) for 3 days.
Morphologies of different cell populations were enumerated by Wright-Giemsa staining of cytospin preparations. Cells were categorized as either immature blasts, intermediate monocytes, or mature macrophages; the relative proportions of each category are shown. Data are means ± standard deviations of three independent clones of each virus construct and are from a representative experiment that was repeated three times.