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Percent relative abundances of species in each M stage compared by quantification method. Species were separated according to Treponema species (A) and non-Treponema species (B). Relative abundances were compared between deep amplicon sequencing of the V3-V4 hypervariable region of the 16S rRNA gene and species-specific qPCR quantification. Fusobacterium sp. targeted by qPCR and F. mortiferum identified by deep amplicon sequencing were both labeled as Fusobacterium sp.
