FIG 3.

Gene flux contributed to the diversity of IncI1 plasmids. (a to c) Maximum likelihood tree of pST26 IncI1 plasmids from this study (n = 36) constructed using complete plasmid DNA sequences (a), core genes (b), and accessory genes (c). The GTR+I, GTR, and JC+I models of nucleotide substitution and the GAMMA model of rate heterogeneity were used for sequence evolution prediction for a, b, and c, respectively (tree was rooted with the closest relative; GenBank CP016585), found through NCBI BLASTn search. Clade numbering was assigned arbitrarily to show the number of clades found. Numbers shown next to the branches represent the percentage of replicate trees where associated isolates cluster together based on ∼100 bootstrap replicates.