Figure 3.

SNHG12 sponges miR-218-5p. (a) The subcellular location of SNHG12 in HUVECs was detected by cell cytoplasm/nucleus fraction analysis. (b) The enrichment of miRNAs in the biotinylated SNHG12 was detected by RT-qPCR. (c) The enrichment of SNHG12 in the biotinylated miR-218-5p was assessed by RT-qPCR. (d) Predicted binding site of SNHG12 to miR-218-5p. (e) Luciferase activity was determined in HUVECs and HEK293T cells co-transfected with NC mimics or miR-218-5p mimics and SNHG12-Wt or SNHG12-Mut. (f) The miR-218-5p level in AS patients’ sera (n = 30) and normal sera (n = 30) was determined by RT-qPCR. (g) MiR-218-5p expression in HUVECs treated with ox-LDL. (h) MiR-218-5p expression in HUVECs transfected with sh-SNHG12. ^*P < 0.05, **P < 0.01, ***P < 0.001