Figure 4.

SNHG12 regulates ox-LDL-induced injury in HUVECs by miR-218-5p. Ox-LDL-treated HUVECs were transfected with sh-NC, sh-SNHG12, sh-SNHG12 + NC inhibitor, or sh-SNHG12 + miR-218-5p inhibitor. (a) MiR-218-5p expression was determined by RT-qPCR. (b) Cell proliferation was detected by CCK-8. (c) The levels proliferation-related proteins (PCNA and Cyclin D1) were detected by western blotting. (d and e) Cell cycle and apoptosis were measured by flow cytometry. (f) Cell apoptosis was estimated by TUNEL. (g and h) The levels of IL-6 and TNF-α were measured by ELISA and western blotting. *P < 0.05, **P < 0.01, ***P < 0.001