Figure 5.

IGF2 is targeted by miR-218-5p. (a) Potential mRNAs of miR-218-5p shown by Venn diagram. (b) The mRNA expression in HUVECs transfected with miR-218-5p mimics. (c) Predicted binding site of IGF2 to miR-218-5p. (d) The interaction of IGF2 with miR-218-5p was validated by luciferase reporter assay. (e and f) IGF2 expression in HUVECs transfected with miR-218-5p mimics, miR-218-5p inhibitors, or control was measured by RT-qPCR and western blotting. (g) The IGF2 level in AS patients’ sera (n = 30) and normal sera (n = 30) was determined by RT-qPCR. (h and i) IGF2 expression in HUVECs treated with ox-LDL. *P < 0.05, **P < 0.01, ***P < 0.001