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. 2021 Jul 21;20(16):1589–1602. doi: 10.1080/15384101.2021.1954758

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Figure 5. — Upregulated miR-641 mitigates circSETD3 effects in bladder cancer cells

(a) Expression of miR-641 was determined by RT-qPCR after upregulation of miR-641. Upregulation of miR-641 or circSETD3. Then, (b and c), Cell viability was determined using CCK-8 assay. (d and e) Cell colony formation was calculated by colony formation assay. (f and g) Cell migration ability was analyzed by transwell assay. (h) The numbers of sphere were determined by microsphere formation assay. (i–k) Protein levels of E-cadherin, N-cadherin, and vimentin were detected by western blotting. Expression of ALDH1, OCT4, and CD133 was detected by RT-qPCR. **P < 0.01, ***P < 0.001.