Figure 6.

MiR-641 directly targets with PTEN

(a) Potential binding sties of PTEN at miR-641 were illustrated. (b) The luciferase activity was measured by dual-luciferase reporter gene assay. (c–e) MRNA and protein expression of PTEN were detected by RT-qPCR and western blotting. (f) and g. Expression of PTEN in matched BLCA tissues and normal tissues (n = 45) was detected by RT-qPCR. (h) Kaplan-Meier survival curve of high and low PTEN expression of BLCA patients. (i) Correlation analysis between PTEN expression and miR-641 expression in BLCA tissues was determined by Pearson correlation analysis. ***P < 0.001.