Table 3.

Body Fat Data for KO Lines with Significantly Increased Body Fat by HTS Only That Supports an External KO Line with High Body Fat

	Normalized % Body Fat						Normalized Body Fat (g)
	Chow		HFD		Pooled Chow and HFD Data		Chow	HFD	Pooled Chow and HFD Data
		KO/WT		KO/WT
KO Line	Mean	(n/n)	Mean	(n/n)	KO	WT	Mean	Mean	KO	WT
Adcy3a	186	10/4	NM	NM	186 ± 84**†	100 ± 12	240	NM	240 ± 184*†	100 ± 22
Aoc3	120	8/4	NM	NM	120 ± 21	100 ± 5	133	NM	133 ± 27*†	100 ± 4
Apln	121	8/4	125	6/4	122 ± 22*	100 ± 20	124	142	131 ± 35*	100 ± 36
Ccn5	129	8/4	120	6/6	125 ± 25*	100 ± 31	125	108	118 ± 29	100 ± 43
Ddah1	106	8/4	134	8/8	134 ± 16*b	100 ± 40	116	150	133 ± 45b	100 ± 44
Enox1	116	8/4	126	6/5	120 ± 23*	100 ± 15	127	128	127 ± 36	100 ± 18
Herc1	106	8/4	188	8/8	147 ± 49**†	100 ± 23	106	235	169 ± 80*†	100 ± 30
Kdm3a	150	8/4	NM	NM	150 ± 24**	100 ± 25	162	NM	162 ± 42*	100 ± 36
Ncoa1	110	8/4	120	8/8	115 ± 20	100 ± 22	119	130	125 ± 30*	100 ± 32
Ncs1	119	8/4	131	6/6	124 ± 35*	100 ± 28	128	148	137 ± 63	100 ± 38
P2rx6	131	8/4	NM	NM	131 ± 27*	100 ± 7	162	NM	162 ± 51*†	100 ± 4
Pnpla2	121	8/8	111	8/8	121 ± 20*†c	100 ± 7	124	104	124 ± 30*†c	100 ± 18
Prmt7	149	8/4	128	8/8	138 ± 32**	100 ± 19	166	124	145 ± 57*†	100 ± 27
Retsat	132	8/4	119	8/8	125 ± 32*	100 ± 26	148	111	130 ± 51	100 ± 38
Rgs10	131	8/4	NM	NM	131 ± 19*†	100 ± 4	150	NM	150 ± 43*†	100 ± 15
Tnfsf13b	126	8/4	NM	NM	126 ± 28	100 ± 13	141	159	141 ± 36*†	100 ± 16
Usp38	142	8/4	133	8/7	138 ± 33**	100 ± 21	159	140	149 ± 52**	100 ± 28

Notes: KO mice different from WT mice, *P < 0.05; **P < 0.01; ^† Statistical analysis by Mann–Whitney test. ^aKO data represent data pooled from 2 KO and 8 HET Adcy3 mice. ^bHFD data only. ^cChow data only.

Abbreviations: HFD, high fat diet; (g), grams; KO, knockout; WT, wild-type; n, number of mice; NM, not measured.