Figure 2.
P53 directly binds to WT1. (a) Endogenous WT1 protein was immunoprecipitated from two human AML cell lines (i.e. HL-60 and KASUMI-1), followed by western blot to detect p53. Normal rabbit IgG was used as a negative control; (b) Endogenous WT1 protein was immunoprecipitated from mouse ESCs or BMCs cells, followed by western blot to detect p53. Normal rabbit IgG was used as a negative control; (c) Wild-type p53 and p53R248Q were co-expressed with WT1 in HEK293T cells. Protein–protein interaction was examined by IP-western using the indicated antibodies