Figure 3.

WT1 recruits p53 to its target genes promoter region. (a) p53 was transiently expressed either singularly or with WT1 in HEK293T cells. The occupancy of p53 at the promoter regions of WT1-target genes was determined by ChIP-qPCR. Mouse IgG was included as negative controls for ChIP-qPCR; (b) HL-60 cells were transduced with retrovirus expressing different shRNAs against WT1. The occupancy of p53 on the promoter regions of indicated WT1-target genes was determined by ChIP-qPCR. Mouse IgG was included as a negative control. Shown are average values of triplicated results with SD; *p < 0.05; **p < 0.01; ***p < 0.001 for the indicated comparison