Figure 4.

piR-651 overexpression promotes the expression of oncogenes. PcDNA-piR-651 and piR-651 shRNA were transfected into MDA-MB-231 cells for 24 h. (a) Western blotting was used to detect the protein levels of MDM2, CKD4 and CyclinD1 in MDA-MB-231 cells. (b) The quantitative analysis of MDM2 protein level. (c) The quantitative analysis of CKD4 protein level. (d) The quantitative analysis of CyclinD1 protein level. Data were presented as mean ± SEM, (N = 4). The difference of the samples between the two groups were analyzed with independent sample t test. The analysis of variance (ANOVA) test was used to evaluate multigroup comparisons of the means