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Chronic jetlag protocol and pancreatic RNA isolation schema. Chronic jetlag (CJ) protocol (A), whereby the light-dark (LD) cycle is characterized by 12 h of light followed by 12 h of dark that is shifted forward 4 h every 2−3 days. Following 4 wk of CJ, wild-type C57BL/6J mice were euthanized every 4 h (B) for 48 h—representing two complete periods.
