Figure 6.

Western blot analysis of placental endothelial nitric oxide synthase (eNOS) S1176 phosphorylation. On gestation day (GD) 14, the reduced uterine perfusion pressure (RUPP) procedure was performed on a subset of normal pregnant (NP) rats. On GDs 15 and 18, vehicle or 10 μg/kg of anti-IFNγ antibody was injected intraperitoneally in a subset of NP and RUPP rats. On GD 19, placentas were collected following euthanization and processed for analysis of placental eNOS expression. A: quantified fold change of placental eNOS expression normalized to β-actin. B: a representative blot of eNOS pS1176 and β-actin expression in all groups is shown. NP: n = 5 rats; RUPP: n = 6 rats; NP + anti-IFNγ: n = 5 rats; RUPP + anti-IFNγ: n = 6 rats. All data are expressed as means ± SEM. Statistical analyses were performed using two-way ANOVA with multiple comparisons followed by Tukey’s post hoc test. ++P < 0.01.