Figure 6.

MitoQ blocks diaphragm myosin heavy chain loss induced by sepsis. A representative Western blot for diaphragm myosin heavy chain (MHC) levels is shown in A, whereas group mean data for MHC protein densitometry levels are presented in B (n = 4 animals/group). CLP significantly reduced diaphragm levels of MHC but administration of MitoQ blocked loss of this critical contractile protein induced by sepsis (P < 0.01 for comparison of MHC content for CLP compared with the other three groups). Densitometry data are presented in box and whisker plots with overlapping individual data points. The box represents 1 SE above and below the mean, the mean is shown by the black line in the box, and the whiskers represent the 5%–95% confidence intervals. ANOVA was used to compare variables across groups treated with different agents; *statistical significance. CLP, cecal ligation puncture; MitoQ, mitoquinone mesylate.