Figure 3.

Histological sections stained with cresyl violet and IBA-1 antibodies. Immunohistochemistry to recognize IBA-1 was performed to provide a marker for microglia; tissues were counterstained using cresyl violet to enable visualization of neuronal morphology. The boxed areas in the left (indicated by i and ii) are shown at a higher magnification in the middle and right. Within those panels, additional higher magnification views are provided in the insets. The circle in Ai indicates the approximate location of the hypoglossal motor nucleus. Neuronal soma were larger in the lumber spinal cord as compared to the more rostral locations. IBA-1 staining was prominent in white matter in the medulla, cervical, and thoracic cord (e.g., arrows in Aii and Bii; inset panels in Bii and Cii). Calibration bars: A–D left: 1 mm, middle (i): 500 µm, inset: 100 µm; right (ii): 500 µm, inset: 100 µm.