Figure 7.

Diaphragm histology indicates profound pathology with apparent loss of myofiber protein. The tissues in A were stained for Myosin-1 (red) and Myosin-2 (brown). The most striking observation is the complete lack of stain in many putative myofibers. These “ghost fibers” are seen to have a cell membrane but do not stain for Myosin. B shows the results of PAS staining to recognize glycogen. Calibration bars: A, left: 100 µm; right: 50 µm; B, left: 200 µm; right: 50 µm. PAS, periodic acid-Schiff.