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. 2021 Aug 23;6(16):e150796. doi: 10.1172/jci.insight.150796

Figure 2. Effect of CD47 expression on cone survival.

Figure 2

(A) Schematics of AAV vectors. AAV8-RedO-FLEX-CD47 is a flip-excision (FLEX) vector in which the CD47 transgene is inverted and flanked by lox2272 (black triangles) and loxP (white triangles) sites. (B) Immunostaining for CD47 in P40 wild-type (CD-1) retinas following infection with AAV8-RedO-GFP or AAV8-RedO-GFP plus AAV8-RedO-CD47. Nuclei were labeled with DAPI. Scale bars: 50 μm. (C) Representative flat mounts of P50 rd1 retinas following infection with AAV8-RedO-GFP, AAV8-RedO-GFP plus AAV8-RedO-CD47, or AAV8-RedO-GFP plus AAV8-RedO-FLEX-CD47. Paired images depict low- and high-magnification views. Scale bars: 1 mm. (D) Quantification of GFP-positive cones in central retinas of rd1 mice (n = 11–17) following infection with AAV8-RedO-GFP, AAV8-RedO-GFP plus AAV8-RedO-CD47, or AAV8-RedO-GFP plus AAV8-FLEX-RedO-CD47. Data are shown as mean ± SEM. ****P < 0.0001 by 2-tailed Student’s t test.