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. 2021 Aug 23;6(16):e148028. doi: 10.1172/jci.insight.148028

Figure 6. Overexpression of Pla2g4a in CT26 induced CD39+γδ Tregs using orthotopic murine models of colorectal cancer.

Figure 6

(A) Approximately 3–4 weeks after CT26-Pla2g4a and CT26-Vec cells were surgically transplanted in the caecal submucosa of BALB/c mice, bloody ascites occurred, and the abdomen increased substantially in the CT26-Pla2g4a group. n = 6 per group. (B) The orthotopic tumor and metastatic tumor in the colon. (C) The tumor weight was robustly increased in the CT26-Pla2g4a group. (D) Representative flow cytometric analysis of CD39+γδ Tregs. (E) The frequency of CD39+γδ Tregs infiltrated in the orthotopic tumors in the 2 groups. (F) Representative H&E staining of graft cross-section in orthotopic tumor. (G) Schematic of SCID mice experimental design. n = 3 per group. (H) The tumor size was shown. (I) The weights of orthotopic tumors. (J) Representative histogram plot of CD39 expression on γδ T cells in each group by flow cytometry. (K) The frequency of CD39 positive cells. M, mucosa; me, muscolaris externa; sm, submucosa. Data shown are representative of 3 independent experiments. Data are shown as mean ± SEM. Unpaired 2-tailed t test was used to compare variables. **P < 0.01, ***P < 0.001. Scale bars: 50 μm.