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. 2021 Aug 9;6(15):e137937. doi: 10.1172/jci.insight.137937

Figure 6. EV isolation from MEC1 and OSU-CLL in standard flasks (cultured in AIM V or RPMI) or CLF (cultured in RPMI).

Figure 6

(A) Western blot analysis of 4 sets of MEC1 EVs cultured in different media (AIM V or RPMI) in standard flasks and CLF isolated by Opti-CUC in absence (-) or presence (+) of initial trehalose. (B) Bead-based flow cytometry analysis of EV isolates from MEC1 and OSU-CLL CLFs in absence (-) or presence (+) of initial trehalose. Delta median fluorescence of samples calculated by subtracting MFI of each sample from its isotype control (n = 6, 2-way ANOVA). Data are represented as mean ± SD.