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. 2021 Aug 9;6(15):e144068. doi: 10.1172/jci.insight.144068

Figure 1. In vitro characterization of EHTs.

Figure 1

(A) Equipment used to make silicone mold and to generate EHTs. (B) Macroscopic image of EHT just prior to grafting. (C) Immunostained EHT (troponin T abbreviated as TNNT2; fibroblasts; vimentin) 28 days after generation. Scale bar: 1000 μm. (D) Higher magnification image of C (TNNT2, fibroblasts, vimentin). Scale bar: 20 μm. (E) Graph of late EHT calcium transient (paced 1 Hz) and calcium transient differences between early and late EHTs (early, <2 weeks; late, >4 weeks). (F) Troponin T cell alignment data (n = 4 early; n = 8 late) and contraction kinetics over time (n = 6). Data are presented as mean ± SEM, and 2-way ANOVA and unpaired Student’s t test were the statistical methods used. *P < 0.05, **P < 0.01, and ***P < 0.001.