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. 2021 Jul 22;6(14):e137708. doi: 10.1172/jci.insight.137708

Figure 8. mTORC1 activation impairs release of the surfactant secretagogue extracellular ATP in response to volutrauma.

Figure 8

(A) SAECs were subjected to volutrauma (24% stretch, 0.2 Hz, 30 min) or static culture (control) prior to measuring extracellular ATP. Data normally distributed, analyzed by Student’s t test, n = 6 wells/condition. (B) HBE cells were subjected to volutrauma (24% stretch, 0.2 Hz, 30 min) or static culture (control) prior to measuring extracellular ATP. Data normally distributed, analyzed by Student’s t test, n = 6 wells/condition. (C) SAECs on polyacrylamide gels were subjected to atelectrauma or control for varying amounts of time prior to measuring extracellular ATP. Data normally distributed, analyzed by 1-way ANOVA with Sidak’s post hoc test, n = 4 for all time points except control n = 3. (D–F) HBE cells were treated with Torin 2 (D) (10 nM), rapamycin (E) (10 nM), SCH772984 (F) (10 μM), or appropriate vehicle control 1 hour prior to in vitro volutrauma. ATP concentration was measured in media following 30 minutes of injurious stretch. Data normally distributed, analyzed by Student’s t test, n = 6 wells/group. Box blots show median ± interquartile range and whiskers define min and max values. *P < 0.05 versus all groups.