Figure 2. Splenomegaly and T cell activation are reduced in IRF5^ΔB mice.

All analyses were done in 5-month-old FcγRIIB^−/−Yaa mice. (A) Spleen weights from WT (n = 10), mb1cre (n = 13), IRF5^F/+ (n = 16), IRF5^ΔB (n = 8), and IRF5^+/– (global heterozygous deletion, n = 5) mice. (B) Splenic cell counts from WT (n = 5), mb1cre (n = 5), IRF5^F/+ (n = 14), and IRF5^ΔB (n = 8) mice. (C) Representative flow cytometry plots of CD4⁺CD62L^–CD44⁺ (effector/memory) and CD62L⁺CD44^– (naive) T cells from spleen of IRF5^F/+ and IRF5^ΔB mice. (D and E) Percentage and number of CD62L^–CD44⁺ CD4⁺ T cells from WT (n = 5), mb1cre (n = 5), IRF5^F/+ (n = 15), and IRF5^ΔB (n = 8) mice. (F and G) Percentage and number of CD62L⁺CD44^–CD4⁺ T cells. (H) Representative flow cytometry plots of CD8⁺CD62L^–CD44⁺ (effector/memory) and CD62L⁺CD44^– (naive) T cells from spleen of IRF5^F/+ and IRF5^ΔB mice. (I and J) Percentage and number of CD62L^–CD44⁺CD8⁺ T cells from WT (n = 5), mb1cre (n = 5), IRF5^F/+ (n = 15), and IRF5^ΔB (n = 8) mice. (K and L) Percentage and number of CD62L⁺CD44^–CD8⁺ T cells. Data are shown as mean ± SEM and were analyzed using 1-way ANOVA with Tukey’s post hoc test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. IRF5, IFN regulatory factor 5.