Figure 3. iLEC^Kras mice develop ectopic lymphatics in bone.

(A) Schematics of the Prox1-CreER^T2 and Kras^LSL-G12D alleles. (B) Schematic showing when mice were fed tamoxifen (2 μl of 25 mg/ml solution). Femurs and tibias were collected when mice were 8 weeks old. (C–F) Bones from iLEC^Ctrl mice (n = 10; C and D) and iLEC^Kras mice (n = 3; E and F) were stained with antibodies against Lyve1 and podoplanin. Bones from iLEC^Ctrl mice did not contain lymphatics. In contrast, bones from 2 of 3 iLEC^Kras mice contained lymphatics (arrows). Bones from iLEC^Ctrl and iLEC^Kras contained numerous podoplanin-positive osteocytes (arrowheads). Scale bar: 100 μm.