Figure 8.

Fertility assessment of Pdyn-Cre/Kiss1^fl/fl knockout (KO) male mice. A: beginning at reproductive maturity, each virgin wild type (WT) or Pdyn-Cre/Kiss1^fl/fl KO male was paired with one proven, WT female for 10 days minimum before replacing the female with another proven, WT female. Individual, representative mice are shown in the timeline. Gray thick marks indicate timepoints of litter births, with gray numbers in parentheses above indicating the number of live pups in that litter. Reference timeline is shown beneath all WT and KO individual fertility timelines (WT n = 3, Pdyn-Cre/Kiss1^fl/fl KO n = 3). B: Pdyn-Cre/Kiss1^fl/fl KO males demonstrate variable, disrupted fertility with diminished, but no statistically significant difference in litter size (unpaired Student’s t test, WT n = 13, Pdyn-Cre/Kiss1^fl/fl KO n = 6) and reproductive success rate, which decreased to 50% (chi-square test, P = 0.1675 KO vs. WT, WT n = 5, Pdyn-Cre/Kiss1^fl/fl KO n = 3).