Figure 4.

microRNA mimic-29a, b, and c transfection did not alter SMCT-1 mRNA levels in intestinal epithelial cells. RNA was extracted from Caco-2 (A) and T-84 (B), 48 h posttransfection with mimics of microRNAs 29a, b and c that target SMCT-1 3′-UTR. SMCT-1 mRNA levels were measured by quantitative real-time PCR as described in materials and methods. Values of mRNA levels for SMCT-1 were normalized against GAPDH mRNA levels. Results are means ± SEM of four independent experiments. SMCT-1, sodium-coupled monocarboxylate transporter-1; UTR, untranslated region.