Figure 5.

mimic-29a, b, and c transfection decreased SMCT-1 protein expression. Cell lysates of Caco-2 (A) and T-84 (B) transiently transfected with control negative and mimic-29a, b, and c were subjected to 10% SDS-PAGE followed by transfer to nitrocellulose membrane. The blot was probed with anti-SMCT-1 or anti-GAPDH antibody. Densitometric analysis of the relative band intensities using ImageJ. Results represent means ± SEM of five different experiments. *Significant differences between control negative vs. mimic-29a, b, and c transfected groups (*P < 0.05). SMCT-1, sodium-coupled monocarboxylate transporter-1.