Figure 7.

Mutating miR-29a, b, and c binding region in 3′-UTR of SMCT-1 abolished the effects of its effects on relative luciferase activity. A: miR-29a, b, and c binding region on SMCT-1 3′-UTR is denoted in red color and respective mutated sequence is in blue color. B: relative luciferase activities in transiently cotransfected cells with miR-29a, b, and c along with 3′-UTR of SMCT-1 or mutated 3′-UTR of SMCT-1. Results are means SEM of four independent experiments. Significant difference between pmirGLO-3′-UTR of SMCT-1 negative control and pmirGLO-3′-UTR of SMCT-1 mimic-29a and c (**P < 0.01) and mimic-29b (***P < 0.001). SMCT-1, sodium-coupled monocarboxylate transporter-1; UTR, untranslated region.