Figure 4.

Muc5b gene expression and protein secretion do not fully correlate with other measures of injury and fibrosis. Muc5b protein in bronchoalveolar lavage fluid (BALF) (A and C, Supplemental Fig. S5, top row) and whole lung gene expression (B and D, Supplemental Fig. S5, bottom row) vary at baseline and after injury among mouse strains and their correlation with percent affected area in injured lung (F and G, correspondingly). Hierarchical clustering of mouse strains based on %changes in Muc5b gene expression, protein, and measures of lung fibrosis (E). n = 8–13 mice were analyzed after bleomycin-induced injury and compared with n = 5–7 saline treated-control mice from each strain.