Table 2.

Offspring anthropometric and organ assessments

	1 Mo				3 Mo				7 Mo				ANOVA	ANCOVA
	Air	E-cig0	E-cig18	ANOVA P=	Air	E-cig0	E-cig18	ANOVA P=	Air	E-cig0	E-cig18	ANOVA P=	Exposure/Age Interaction P=	Exposure/Sex Interaction P=
Data at birth/weaning
Pups in study (n=)	5	11	13		9	9	7		7	7	7
Sex (male, female)	3, 2	6, 5	6, 7		4, 5	5, 4	3, 4		3, 4	2, 5	4, 3
Pup body mass at P21, g	44.9 ± 0.7	43.5 ± 2.2	44.4 ± 2.1	0.91	42.5 ± 2.8	41.2 ± 3.5	40.0 ± 3.4	0.89	37.9 ± 2.0	40.9 ± 3.8	39.5 ± 3.9	0.94	0.97	0.53
Data at sacrifice
Body mass, g	184 ± 23	193 ± 20	280 ± 50	0.59	388 ± 47	395 ± 47	360 ± 33	0.86	519 ± 85	429 ± 70	517 ± 62	0.61	0.74	0.47
Heart mass, g	0.69 ± 0.04	0.73 ± 0.04	0.75 ± 0.10	0.89	1.13 ± 0.10	1.13 ± 0.10	1.20 ± 0.06	0.84	1.07 ± 0.24	1.19 ± 0.10	1.43 ± 0.18	0.37	0.71	0.79
Heart/body mass ratio, mg/mg	4.4 ± 0.2	4.2 ± 0.2	3.7 ± 0.1*	0.03	3.0 ± 0.1	3.1 ± 0.2	3.4 ± 0.1	0.23	2.6 ± 0.3	3.2 ± 0.1*	2.7 ± 0.1	0.09	0.01	0.47
Lung, g	1.04 ± 0.11	1.05 ± 0.10	1.15 ± 0.14	0.79	1.41 ± 0.10	1.49 ± 0.13	1.76 ± 0.15	0.15	1.70 ± 0.05	1.42 ± 0.08	1.72 ± 0.10	0.06	0.52	0.55
Lung/body mass ratio, mg/mg	6.9 ± 1.2	6.2 ± 0.8	5.9 ± 0.3	0.65	3.8 ± 0.3	4.2 ± 0.4	4.9 ± 0.3	0.09	3.9 ± 0.9	4.2 ± 0.2	3.8 ± 0.3	0.87	0.43	0.28
Liver mass, g	7.0 ± 0.6	8.2 ± 0.7	9.0 ± 1.6	0.62	13.2 ± 1.5	12.9 ± 2.0	11.5 ± 1.5	0.78	12.9 ± 3.0	14.1 ± 2.3	16.2 ± 4.2	0.79	0.83	0.89

Values are means ± SE, unless indicated otherwise. ANCOVA, analysis of covariance; E-cig0, E-cig device without nicotine; E-cig18, E-cig device with 18 mg/mL nicotine; P21, postnatal day 21 (i.e., weaning).

*P < 0.05 compared with control (i.e., air) in same age group.