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αMDG uptake by WT and Q457R mutant SGLT1. Oocytes were injected with 50 nl (1 µg/µl) of cRNA coding for WT or Q457R mutant SGLT1. After 3 days, uptake of 50 µM 14C-αMDG was measured. Values represent the mean of 8 oocytes and the error bars indicate the standard error. Q457R mutant uptake was not different from uptake by NI oocytes, 1.7% of the WT uptake (3 vs. 180 pmoles/h/oocyte).
