Figure 5.

(Freeze-fracture) P fracture face of the plasma membrane of a NI oocyte and oocytes injected with Q457R or WT cRNA. NI oocyte (A) showed 7-nm-diameter particles in the P-face at a density of 200/mm², whereas in WT injected oocyte (B), the density of particles increased to 1000/mm². Similar density was obtained in Q457R cRNA injected oocytes (C). Q457R injected oocyte is the same as the one in Figures 7B and 8A. The 25 mM Na⁺/glucose current was −800 nA at –50 mV for the WT oocyte and 0 for Q457R (Figure 7); the SGLT1 charge movements were 8.5 and 3.7 nC (Figure 8A). Scale bar 200 nanometers.