Figure 6.

Immunolocalization of SGLT1 protein in human small intestine of a normal (control) (A) and a GGM patient (9) with the Q457R missense mutation (B). Double staining procedures shows SGLT1 (red) as a sharp line localized to the brush border of the enterocytes and the basal nucleus stained in blue. Goblet cells between the enterocytes were unstained for SGLT1. There were no differences in location of SGLT1 in enterocytes from the normal and the GGM (magnification x125). In a separate case of GGM, C292Y, the mutant protein did not reach the brush border membrane and was trapped just above the nuclei.¹⁴ Scale bar 30 micrometers.