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Collection of intestinal lavages on day 35 from three mice/per groups and measurement of immune responses against vaccine regimens by enzyme-linked immunosorbent assay; intestinal lavage total immunoglobulin G (panel A) and intestinal lavage immunoglobulin A (panel B); statistically significant difference in antibody responses between immunized mice and non-mmunized mice (*P<0.05; **P<0.001); mean values±standard deviation of three independent experiments (Ref. V. cholerae)
