Table 2.
Antibodies and Conditions for Immunohistochemistry.
| Antigen | Antibody | Dilution | Incubation | Source | Catalog Number | Retrieval Conditions |
|---|---|---|---|---|---|---|
| Citrullinated histones | Rabbit polyclonal | 1 to 200 | 4°C overnight | Abcam | ab5103 | Citrate pH 6.1, heat-induced |
| Eosinophil peroxidase | Mouse monoclonal (MM25-82.2.1) | 1 to 1000 | 32 min, RT | Dr Lee (Mayo Clinic, AZ) | NA | Leica Protocol |
| Myeloperoxidase | Rabbit polyclonal | 1 to 1000 | 32 min, RT | Dako | A0398 | Roche Protocol |
| PAR-2 | Mouse monoclonal (clone SAM11) | 1 to 400 | 4°C overnight | Abcam | ab184673 | Citrate pH 6.1, heat-induced |
| p-ERK | Rabbit monoclonal (clone D13.14.4E) | 1 to 500 | 32 min, RT | Cell Signaling | mAb#4370 | EDTA-based pH 9.0, heat-induced |
| Tissue factor | Rabbit polyclonal | 1 to 400 | 32 min, RT | ThermoFisher | PA5-27278 | EDTA-based pH 9.0, heat-induced |
NA = not applicable; p-ERK = phospho-extracellular signal-regulated kinase; PAR-2 = protease-activated receptor-2; RT = room temperature.