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A. A drop of 10% agarose is applied to a glass slide between the two double-taped spacers by using a micro spatula. B. A slide glass is placed on the 10% agarose drop. C. A drop of microsphere suspension and a drop of serotonin/M9 solution are applied to the agarose pad on the stage of a stereo microscope. D. A sample ready for imaging, in which the cover glass is stabilized by VALAP on the agarose pad (a non-injected worm is immobilized in this image).
