Table 3.
Summary of intraday and interday precisions, accuracies, extraction recoveries, and matrix effects of the four analytes in rat plasma (n = 6).
| Analytes | Concentration (ng/mL) | Intraday RSD (%) | Interday RSD (%) | Accuracy (RE%) | Recovery (%) | Matrix effect (%) |
|---|---|---|---|---|---|---|
| G-Rh1 | 25 | 9.43 | 9.69 | 0.58 | 90.65 ± 2.34 | 95.67 ± 2.45 |
| 250 | 0.95 | 0.49 | 3.84 | 93.67 ± 4.08 | 87.37 ± 8.45 | |
| 2500 | 1.21 | 2.95 | 4.08 | 96.59 ± 0.62 | 97.57 ± 3.11 | |
|
| ||||||
| G-Rb1 | 25 | 6.65 | 6.21 | −3.47 | 86.06 ± 2.57 | 91.33 ± 0.96 |
| 250 | 0.98 | 4.56 | −1.44 | 91.86 ± 2.25 | 94.22 ± 1.47 | |
| 2500 | 3.50 | 2.38 | 2.38 | 94.19 ± 1.55 | 95.55 ± 1.22 | |
|
| ||||||
| G-Rc | 25 | 5.35 | 6.21 | −7.44 | 89.46 ± 5.13 | 89.42 ± 3.17 |
| 250 | 4.38 | 3.28 | 6.34 | 91.28 ± 4.56 | 94.43 ± 5.85 | |
| 2500 | 3.86 | 4.61 | −6.51 | 93.60 ± 2.02 | 96.85 ± 7.33 | |
|
| ||||||
| G-Rd | 25 | 6.29 | 4.82 | −8.92 | 87.64 ± 4.87 | 91.78 ± 5.38 |
| 250 | 1.65 | 2.20 | −5.12 | 90.87 ± 0.56 | 96.65 ± 3.97 | |
| 2500 | 3.87 | 4.51 | 7.70 | 96.86 ± 5.08 | 89.94 ± 3.77 | |