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. 2021 Mar 17;35(9):2635–2649. doi: 10.1038/s41375-021-01201-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A VEGF-A and angiogenin were quantified in supernatants (SUPS) from FL monocultures and FL-M2 co-cultures (48 h; left panels). Those supernatants were also used in a HUVEC tube formation assay (right panels). The number of nodes and junctions per field were obtained using ImageJ software with Angiogenesis Analyzer plugin. The data is representative of 3 FL patients. B Surface expression of CD29 (Integrin beta-1, ITGB1) and CD18 (Integrin beta-2, ITGB2) was analyzed by flow cytometry in FL primary samples (n = 7) after 48 h of culture with or without M2 (left panels). FL cells from FL monocultures or FL-M2 co-cultures (48 h) were subjected to an adhesion assay to VCAM or Fibronectin (FN) (n = 5) (right panel). C MMP9 vas quantified in supernatants (SUPS) from FL monocultures or FL-M2 co-cultures (n = 5) and the corresponding FL cells were recovered and subjected to an invasion assay for 24 h.