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. 2021 Aug 4;597(7874):126–131. doi: 10.1038/s41586-021-03794-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a–c, Left, cryo-EM densities for the modelled regions of the eugenol-bound structure (a), the DEET-bound structure (b), and the apo structure (c). Models are shown in stick representation within the density, with the helices denoted underneath from the N terminus (S0) to the C terminus (S7b). Right, local resolution estimation for each final map, calculated in cryoSPARC v2. Side views shown with front and back subunits removed for visualization. d–f, Top, FSC curves for the final cryo-EM density maps, obtained with cryoSPARC v2. The horizontal dashed line intersects at 0.143, the cutoff value. Bottom, FSC relationships between final map and model (black, sum), half-map 1 and model (red, work), and half-map 2 and model (blue, free), calculated in phenix.mtriage.