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. 2021 Feb 23;35(9):2658–2671. doi: 10.1038/s41375-021-01158-9

Fig. 2. Caspase-1 is activated in BMMNCs in response to BM chemoattractants, and caspase-1-KO BMMNCs show a decrease in chemotaxis.

Fig. 2

A luminescence measured in WT BMMNCs cultured in the absence or presence of ATP (10 µM), S1P (0.1 µM), or SDF (100 ng/ml) for 3 h at 37 °C prior to adding Caspase-1 Glo® 1 reagent. B The number of CFU-GM colonies formed from WT or Casp1-KO BMMNC samples recovered from the lower chamber of a 24-well Costar Transwell plate into which four different sets of chemoattractants, including SDF-1 (5 ng/ml), S1P (0.1 μM), ATP (0.25 μg/ml), LL-37 (2.5 μg/ml), or SDF-1 plus LL-37, were added to chemoattract the BMMNCs placed on the upper layer of the cell culture insert through a permeable membrane. The data are presented as means ± SE. Unpaired Student’s t-test was used for the determination of significance (*p ≤ 0.05 and #p ≤ 0.005).