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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Nat Neurosci. 2021 Jul 8;24(9):1235–1242. doi: 10.1038/s41593-021-00879-5

Extended Data Fig. 3. Comparison of in vitro and in vivo identified LADs.

Extended Data Fig. 3

a, Venn diagram showing percent overlap of LAD coverage between brain NPCs and in vitro NPCs (DamID). b, Representative micrographs of DNA-FISH (red) with LaminB ICC (green) in brain NPCs and SVZ-cNSCs for four loci previously annotated as cLADs. Scale bar 2 μm. Multiple images were taken to quantitate sub-nuclear localization of the DNA-FISH loci. c, Quantifications of DNA-FISH for loci shown in b. Percentages indicate loci within 0.5 μm (grey area) from the nuclear lamina. 50–60 nuclei were quantified for each locus. d, LaminB GO-CaRT tracks in brain NPCs, SVZ-cNSCs and in vitro NPCs (DamID) over cLAD DNA-FISH loci (dashed box) shown in b. e, LaminB GO-CaRT tracks of brain NPCs, E13 lung, E13 liver, SVZ-cNSCs and 3T3 MEFs. In vitro NPC and cLAD track (shown on top) are DamID derived6. Dashed box indicates a region where some previously annotated “cLADs” are not observed in tissues in vivo but detected by GO-CaRT in SVZ-NSCs and 3T3 MEFs. f, Venn diagram showing percent overlap of LAD coverage between cLADs and LADs common to brain, lung and liver.