Fig. 3.

a Gating strategy used to analyse markers of differentiation of B regulatory cells. They are identified as CD19+CD24+CD27+ Breg, CD19+CD24+CD38+Breg and CD19+CD1d+CD5+ Breg. b, c Percentages of different CD19+CD24+CD27+ Breg cell subpopulations in controls (n = 10) and patients at baseline (n = 10), obtained by manual gating. Data represent individual values, mean (centre bar) ± SEM (upper and lower bars). CI confidence interval. Mann-Whitney U test and Wilcoxon ranked test were used. p < 0.05 was used for statistical significance