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. 2021 Sep 1;12:5208. doi: 10.1038/s41467-021-25345-5

Fig. 1. Icos responds to simultaneous inputs from several post-transcriptional regulators.

Fig. 1

Naive CD4+ T cells (d0) from tamoxifen-gavaged mice of the indicated genotypes were activated by anti-CD3/anti-CD28 (day1–2) and subsequently cultivated in IL-2 containing media (d4–5). a, d, g, j Bar diagrams show the average of daily flow cytometric measurements of Icos expression with quantified mean fluorescence intensities (MFI) over a 5-day period to analyze changes due to inducible inactivation of Roquin-1 and Roquin-2 (Rc3h1 and Rc3h2), Regnase-1 (Zc3h12a) Wtap or Dgcr8. Significance was calculated using the unpaired t-test (two-tailed) for data from three independent experiments using one mouse per genotype (n = 3). Error bars, mean ± s.d. b, e, h, k Representative histograms of Icos expression at the specified days. c, f, i, l Histograms confirming depletion of the respective target proteins. m–q Western blots showing patterns of dynamic RBP regulation after anti-CD3/CD28 mediated T cell activation (n = 2). significance *p value = 0.01–0.05; **p value = 0.001–0.01; ***p value = <0.001. Calculated p values: (a) 0d p = 0.005, 2d p = 0.016, 3d p = 0.012, 4d p = 0.0005, 5d p = 0.0017, (g) 5d p = 0.009, (j) 4d p = 0.022, 5d p = 0.009. Source data are provided as a Source Data file.