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. 2021 Jul 8;31(9):965–979. doi: 10.1038/s41422-021-00532-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative LC3B western blot and immunofluorescence for the WT versus LC3B-knockout HEK293T cells. Note that different LC3B antibodies were used for western blot and immunofluorescence as indicated in “Material and Methods”. b Representative images and quantifications of the BODIPY493/503 staining of the OA-induced LDs in the WT versus LC3B-knockout HEK293T cells treated with the indicated compounds (5 μM for LD·ATTECs). Scale bar, 20 μm. The LD number per cell and averaged LD size in each field were quantified by ImageJ (particle analysis) in a blinded manner. n indicated the number of independently plated wells from two batches of experiments. ns, P > 0.05; ^$P < 0.0001 by one-way ANOVA (the F and degree of freedom values have been indicated) and Dunnett’s post hoc tests (compared to the DMSO group).