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. 2021 Sep 1;12:5222. doi: 10.1038/s41467-021-25461-2

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A The S. venezuelae (wild-type ftsZ-ypet derivative, MD100) growth curve (in 5 ml culture), performed in n = 3 independent experiments. The mean optical density values and standard deviation (whiskers) are shown on the scheme. The critical time points of sporogenic development are marked with arrows: growth arrest and cessation of DNA replication (red), the appearance of FtsZ ladders (green) and the spore formation (blue). The normalised FtsZ-YPet fluorescence (a marker of a synchronous cell division) [U/µg] and the relative oriC/arm ratio (a marker of DNA replication; n = 3 independent experiments: mean oriC/arm values as well as calculated standard deviations (whiskers) are shown on the diagram) are shown as insets in the plot, with X axes corresponding to the main plot X axis. The oriC/arm ratio at the 26 h of growth was set as 1.0. The right panel shows the representative visualisation of condensing nucleoids (n = 250 analysed nucleoids per a single time point; DNA stained with 7-AAD) and FtsZ-YPet at 22 h of growth; scale bar 2 µm. B The normalised Hi-C contact map obtained for the wild-type (ftsZ-ypet derivative, MD100) after 22 h of growth (in 5 ml culture). The dotted pink lines mark the position of the oriC region. The dotted black lines mark the positions of the left (LTD) and right (RTD) terminal domains. The boundaries of the LTD and RTD are marked directly on the Hi-C contact map with the orange dotted lines. The contact range within the secondary diagonal axis is marked with black dots. C Top panel: the normalised Hi-C contact maps obtained for the wild-type (ftsZ-ypet derivative, MD100) strain growing for 13, 15, 17 and 25 h (in 5 ml culture). Bottom panel: the differential Hi-C maps in the logarithmic scale (log2) comparing the contact enrichment at 22 h of growth (red) versus 13, 15, 17 and 25 h of growth (blue). The X and Y axes indicate chromosomal coordinates binned in 30 kbp. The right panel shows the model of chromosome rearrangement in the course of sporulation. D Top panel: the normalised Hi-C contact maps generated for parA and parB mutants (ftsZ-ypet derivatives MD011 and MD021, respectively) growing for 22 h (in 5 ml culture). Bottom panel: the differential Hi-C maps in the logarithmic scale (log2) comparing the contact enrichment in the wild-type strain (red) versus the mutant strain (blue). The right panel shows the model of chromosome organisation in the parA and parB mutants.